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PRODID:https://murmitoyen.com/events/vanille/udem/
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UID:69dafcc4f3ce1
DTSTAMP:20260411T220036
DTSTART:20180117T110000
SEQUENCE:0
TRANSP:OPAQUE
DTEND:20180117T110000
URL:https://murmitoyen.com/events/vanille/udem/detail/798627-conference-de-
 chimie-avec-la-prof-dajana-vuckovic-concordia
LOCATION:Université de Montréal - Pavillon J.-Armand-Bombardier\, 5155\, 
 chemin de la rampe \, Montréal\, QC\, Canada\, H3T 2B2
SUMMARY:Conférence de chimie avec la Prof. Dajana Vuckovic (Concordia)
DESCRIPTION:Titre: Improving Metabolite Coverage in Untargeted LC-MS Metabo
 lomics
\nEndroit : Pavillon J.-Armand Bombardier\, salle 1035 à 11 h
\
 nHôte : Karen Waldron
\nCette conférence sera prononcée par la Profes
 seure Dajana Vuckovic du département de chimie de l'Université Concor
 dia.
\nRÉSUMÉ: The main objective of metabolomics/lipidomics is the anal
 ysis of all metabolites/lipids present in a particular biological system i
 n order to study dynamic processes involved in an organism’s response to
  normal and abnormal biological or external stimuli. Metabolome effectivel
 y integrates both genetic and environmental inputs\, thus providing the re
 adout closest to the phenotype. This allows us to understand individual di
 fferences in response in unprecedented molecular detail. Mass spectrometry
  (MS) in combination with liquid chromatography (LC) is currently the most
  powerful technique to use for untargeted metabolomics because it provides
  the highest metabolite coverage in a single analysis  However\, current 
 gold standard protocols are capable of detecting only medium to high abund
 ance metabolites\, often yielding no information on biologically-important
  metabolites present in low concentrations. Furthermore\, standard untarge
 ted metabolomics protocols do not provide accurate information for unstabl
 e metabolites leading to erroneous biological interpretation due to analyt
 ical artefacts. My research program focuses on resolving some of these maj
 or challenges in current metabolomics analysis of biological fluids and ti
 ssues by: (i) increasing accuracy and metabolome coverage of  low abundan
 ce metabolome\, and (ii) improving analysis of unstable metabolome such as
  eicosanoids and lipid peroxidation products. In this talk\, I will discus
 s different sample preparation strategies that can be used to improve meta
 bolome coverage in global metabolomics approaches including sequential ext
 raction and new extraction materials such as nanomaterials and ionic liqui
 ds.  I will also discuss briefly  the important role that the mobile pha
 se additives can play to improve electrospray ionization (ESI) efficiency\
 , with particular focus on the usefulness of acetic acid mobile phase addi
 tives for negative ESI. In particular\, I will show the advantages of usin
 g this mobile phase to improve lipid coverage for untargeted lipidomics in
  plasma. Finally\, I will briefly discuss in vivo solid-phase microextract
 ion as a new technique for measuring lipidome in live\, freely moving anim
 als for the first time.
\n
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\nInformations supplémentaires
\nAnnonce PD
 F de la conférence
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