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PRODID:https://murmitoyen.com/events/vanille/udem/
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UID:69e00e3230879
DTSTAMP:20260415T181618
DTSTART:20140428T150000
SEQUENCE:0
TRANSP:OPAQUE
DTEND:20140428T163000
URL:https://murmitoyen.com/events/vanille/udem/detail/407067
LOCATION:Université de Montréal - Pavillon Claire-McNicoll\, 2900\, chemi
 n de la Tour\, Montréal\, QC\, Canada\, H3T 1J6
SUMMARY:Conférence avec le Professeur John Pezacki (CNRC/Ottawa)
DESCRIPTION:Titre : From Nitrone Cyclooctyne Cycloadditions to Activity-Bas
 ed Protein Probes: Chemical Tools for Studying Living Systems.Cette confé
 rence sera prononcée (en anglais) par le Professeur John Pezacki\, qui e
 st à la fois chef de groupe de l'Institut Steacie des sciences molécula
 ires du Conseil national de recherches Canada (CRNC) et professeur auxilia
 ire aux départements de chimie et de Biochimie\, microbiologie & immunolo
 gie de l'Université d'Ottawa.Résumé : We have developed a number of too
 ls for the discovery and characterization of biomolecular interactions inc
 luding new methods in activity-based protein profiling\, activity-based im
 aging\, tools for cell surface labeling\, and small molecule probes for in
 vestigating lipid metabolism and membrane alterations. Representative exam
 ples will be presented. Reactions that do not interfere with biological fu
 nctionality\, termed bioorthogonal\, have emerged as powerful tools for th
 e site specific modification of biomolecules in cells and within living or
 ganisms. We have developed several strategies based on strain promoted cyc
 loadditions of cyclic nitrones with cyclooctynes for bioothogonal labeling
  of proteins. These reactions are among the fastest reported in the litera
 ture and have potential to be used in multiplex labeling as well. Reaction
 s of a series of cyclic nitrones with biaryl-aza-cyclooctynone (BARAC) and
  bicyclo[6.1.0]nonyne (BCN) and their ability to probe biological systems 
 will be presented. We have also developed a number of probes for activity-
 based protein profiling (ABPP)\, a technique which allows for the identifi
 cation of differentially active enzymes in complex proteomic samples. Exam
 ples for the study of fatty acid synthase (FASN)\,  rhomboid proteins\, a
 nd phosphatidylinositol kinases and their application in profiling studies
  will also be presented.  Taken together these studies will be used to il
 lustrate new ways to track down the cellular and molecular details of how 
 biological systems work\, such as how the hepatitis C virus hijacks its ho
 st cells\, discover new biomarkers\, and new targets for therapeutic inter
 vention.Information supplémentaire
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TZID:America/Montreal
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