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UID:69e25ce6cd726
DTSTAMP:20260417T121638
DTSTART:20120413T113000
SEQUENCE:0
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DTEND:20120413T130000
URL:https://murmitoyen.com/events/vanille/udem/detail/110969
LOCATION:Université de Montréal - Pavillon J.-Armand-Bombardier\, 5155\, 
 chemin de la rampe \, Montréal\, QC\, Canada\, H3T 2B2
SUMMARY:Conférence du Professeur Jean-Luc Popot (IBPC Paris)
DESCRIPTION:Titre : Applications of Amphipols to Membrane Protein Studies 
 and Some New Insights Into Membrane Protein Folding.La conférence sera pr
 ononcée par le professeur Jean-Luc Popot de l'institut de Biologie Phys
 ico-Chimique du CNRS et de l'Université Paris Diderot (France). Elle sera
  donnée en anglais.Résumé : Amphipols (APols) are short amphipathic po
 ly­mers designed to substitute to detergents for hand­ling membrane prot
 eins (MPs) in aqueous solutions. Upon trapping a MP with APols\, a non-cov
 alent but stable complex forms\, which is hydrosoluble and in which the pr
 otein is\, in general\, much more stable than in deter­gent solution. In 
 MP/APol complexes\, the surfactant adsorbs onto the hydrophobic trans­mem
 ­brane sur­fa­ce of the protein\, leaving extramembrane surfaces free t
 o interact with water-soluble ligands (cartoon). Functional per­turbation
 s appear to be rare.One of the first APols to be designed\, called A8-35\,
  has been exten­sively stu­died\, as well as the com­­plexes it makes 
 with a number of MPs. A8-35 consists of a relatively short polyacrylate ch
 ain\, part of the carboxylates of which have been randomly grafted with oc
 tylamine and isopropylamine\, making it amphipathic. APols with a dif­­f
 erent chemical structure\, such as sulfonated and non-ionic APols\, have b
 een recently developed. Over the years\, variously labeled and functionali
 zed versions of A8‑35 have been synthesized and validated. Those include
  deuterated APols\, particularly useful for neutron scattering and NMR stu
 ­dies\, fluo­rescent APols\, whose distribution can be easily followed d
 uring fractionation experi­ments and which can be used to carry out FRET 
 studies\, and tagged APols\, which can mediate the attachment of MPs onto 
 solid supports such as chips or beads.The applications of APols that have 
 been validated to date include stabilizing fragile MPs and MP complexes\, 
 solution NMR studies\, electron microscopy\, diagnostics and ligand bindin
 g studies\, folding MPs from a denatured sta­te\, MP cell-free synthesis 
 and vaccination. Various other applications\, e. g. in proteomics\, are c
 urrently being developed. An overview will be presented of those applicati
 ons that appear most ready to be usefully exploited by the membrane protei
 n community. The applications and implications of APol-assisted MP folding
  and cell-free synthesis will be discussed. Information supplémentaire
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