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DTSTAMP:20260417T152542
DTSTART:20120127T113000
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URL:https://murmitoyen.com/events/vanille/udem/detail/96222
LOCATION:Université de Montréal - Pavillon Claire-McNicoll\, 2900\, chemi
 n de la Tour\, Montréal\, QC\, Canada\, H3T 1J6
SUMMARY:To what length will E. coli grow?
DESCRIPTION:Conférence du Dr Ziad El-Hajj\, Postdoctoral Fellow\, Departm
 ent of Biology\, Concordia University. Cette conférence sera prononcée e
 n anglaisRésuméDivision in rod-shaped bacteria such as Escherichia coli 
 is a cycle that involves accumulation of biomass and elongation of the cel
 l\, followed by the activation of the division machinery at midcell and th
 e subsequent septation into two daughter cells. Wild-type E. coli can divi
 de even in medium of quite low osmolality e.g. Luria broth with or without
  salt.We have isolated a mutant strain which grows and divides as 0.5-1 mi
 cron rods  in a medium of high osmolality and elongates to form enormousl
 y long cells (as long as 750 microns) in LB without salt. These mutant cel
 ls accumulate mass during elongation almost as quickly as normal E. coli w
 ould do in the same medium. However they show no trace of constriction\, a
 nd no septum as judged by FtsZ-GFP. Under low osmolality the mutant has re
 duced levels of intracellular FtsZ\, the major septal protein and initiato
 r of cell division\, and as a result the cells elongate without being able
  to initiate a septum. Cell division can be restored by mild overexpressio
 n of FtsZ but not by the later septal protein FtsW. Many of the very long 
 filaments formed by our mutant are  viable and can resume division if shi
 fted to a high osmolality medium. We propose that E. coli relies on a rela
 tively high external osmolality in order to produce a septum and divide\, 
 but that this requirement can be circumvented by a mechanism that is disru
 pted in our mutant. We further suggest that this mechanism involves a post
 -translation modification that stabilizes FtsZ under low osmolality.Confé
 rence présentée par le Département de microbiologie et immunologie de l
 'Université de Montréal. Crédit photo : site Web du Département de mic
 robiologie et immunologie
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