BEGIN:VCALENDAR
VERSION:2.0
PRODID:https://murmitoyen.com/events/vanille/udem/
X-WR-TIMEZONE:America/Montreal
BEGIN:VEVENT
UID:69d9a8f393101
DTSTAMP:20260410T215043
DTSTART:20190315T113000
SEQUENCE:0
TRANSP:OPAQUE
DTEND:20190315T123000
URL:https://murmitoyen.com/events/vanille/udem/detail/845419-high-speed-ato
 mic-force-microscopy-simon-scheuring-weill-cornell-medicine
LOCATION:Université de Montréal - Pavillon Roger-Gaudry\, 2900\, chemin d
 e la Tour\, Montréal\, QC\, Canada\, H3T 1J6
SUMMARY:High-Speed Atomic Force Microscopy - Simon Scheuring (Weill Cornell
  Medicine)
DESCRIPTION:High-Speed Atomic Force Microscopy: A Novel Tool to Study Struc
 ture and Dynamics of Single Unlabeled Membrane Proteins
\nThe advent of hi
 gh-speed atomic force microscopy (HS-AFM(1)) has opened a novel research f
 ield for the dynamic analysis of single bio-molecules: Molecular motor dyn
 amics (2\,3)\, membrane protein diffusion (4)\, assembly (5) and conformat
 ional changes (6) could be directly visualized. Further developments for b
 uffer exchange (7) and temperature control (8) during HS-AFM operation pro
 vide breakthroughs towards the performance of dynamic structural biochemis
 try using HS-AFM. I will exemplify the power of HS-AFM for the quantitativ
 e analysis of function-related structural dynamics on the membrane deforma
 tion complex ESCRT-III (5)\, a glutamate transporter homologue (6)\, and l
 igand-gated ion channels (9\,10). Finally\, I will introduce high-speed AF
 M line scanning (HS-AFM-LS) and high-speed AFM height spectroscopy (HS-AFM
 -HS) that reaches millisecond and microsecond temporal resolution of singl
 e molecule dynamics (11). 
\n 
\nReferences:
\n1) Ando\, Chem Rev 2014\,
  114(6):3120-88
\n2) Kodera\, Nature 2010\, 468(7320):72-6
\n3) Uchihashi\
 , Science 2011\, 333(6043):755-8
\n4) Casuso\, Nat Nanotechnol 2012\, 7(8)
 :525-9
\n5) Chiaruttini\, Cell 2015\, 163(4):866-79.
\n6) Ruan\, PNAS 2017
 \, 114(7):1584-1588
\n7) Miyagi\, Nat Nanotechnol 2016\, 11(9):783-90
\n8)
  Takahashi\, Small 2016\, 12(44):6106-6113
\n9) Ruan\, 2018 115(41):10333-
 10338
\n10) Marchesi\, Nature Communications\, 2018\, 9(1):3978
\n11) Heat
 h and Scheuring\, Nature Communications\, 2018\, 9(1):4983
\nLa conférenc
 e est pour tout public et le café est servi dès 11h30.
\n 
\nhttps://bi
 o-afm-lab.com/director/
END:VEVENT
BEGIN:VTIMEZONE
TZID:America/Montreal
X-LIC-LOCATION:America/Montreal
END:VTIMEZONE
END:VCALENDAR