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PRODID:https://murmitoyen.com/events/vanille/udem/
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BEGIN:VEVENT
UID:69da17048caa0
DTSTAMP:20260411T054020
DTSTART:20181116T113000
SEQUENCE:0
TRANSP:OPAQUE
DTEND:20181116T123000
URL:https://murmitoyen.com/events/vanille/udem/detail/822191-mapping-molecu
 lar-transport-interactions-of-biomolecules-in-cells-via-fluorescence-fluct
 uation-analysis-of-images-paul-wiseman-mcgill
LOCATION:Université de Montréal - Pavillon Roger-Gaudry\, 2900\, chemin d
 e la Tour\, Montréal\, QC\, Canada\, H3T 1J6
SUMMARY:Mapping molecular transport & interactions of biomolecules in cells
  via fluorescence fluctuation analysis of images - Paul Wiseman (McGill)
DESCRIPTION:The transport properties of biomolecules in cells can reveal a 
 great deal about the functional interactions regulating cells at the molec
 ular level. Various biophysical methods have been developed to measure the
 se properties in cells\, although most have relied on fluorescence microsc
 opy imaging as the window for measurement of labeled macromolecules in liv
 ing cells. I will review some of these approaches before describing curren
 t image correlation spectroscopy techniques. Image correlation methods are
  an extension of fluorescence fluctuation spectroscopy that can measure pr
 otein-protein interactions and macromolecular transport properties from in
 put fluorescence microscopy images of living cells. These approaches are b
 ased on space and time correlation analysis of fluctuations in fluorescenc
 e intensity within images recorded as a time series using a fluorescence m
 icroscope. We previously introduced spatio-temporal image correlation spec
 troscopy (STICS) which measures vectors of protein flux in cells based on 
 the calculation of a spatial correlation function as a function of time fr
 om an image time series. Here we will describe the application of time win
 dow STICS and its two color extension\, spatio-temporal image cross-correl
 ation spectroscopy (STICCS)\, for measuring cellular waves of adhesion rel
 ated macromolecules talin and vinculin as well as cytoskeletal actin betwe
 en assembling and disassembling podosomes in dendritic immune cells. Podos
 omes are cylindrical membrane complexes with an integrin adhesive ring and
  an actin rich core that are associated with cellular migration and invasi
 on in specific cell types. EM and super-resolution microscopy of cells sho
 ws radial actin filaments that connect neighboring podosomes so we applied
  pair vector correlation analysis to further characterize the transport wa
 ves within connected podosome clusters. These analyses combined with pharm
 acological perturbation experiments show that podosome turnover is coordin
 ated within local clusters in cells with a correlation length scale extend
 ing to next nearest neighbor podosomes. As well I will introduce future ex
 tensions of ICS including new extensions to super-resolution fluorescence 
 microscopy.
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\nLa conférence est pour tout public et le café est ser
 vi dès 11h30.
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TZID:America/Montreal
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