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DTSTAMP:20260412T200527
DTSTART:20161118T140000
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DTEND:20161118T140000
URL:https://murmitoyen.com/events/vanille/udem/detail/723603-conference-du-
 professeur-andrzej-j-kulik-lausanne
LOCATION:Université de Montréal - Pavillon J.-Armand-Bombardier\, 5155\, 
 chemin de la rampe \, Montréal\, QC\, Canada\, H3T 2B2
SUMMARY:Conférence du Professeur Andrzej J. Kulik (Lausanne)
DESCRIPTION:Titre: Nanoscale Infrared Spectroscopy: applications in biology
  –LHCII proteins and Amyloids
\nEndroit : Pavillon J.-Armand Bombardier\
 , salle 1035 à 14 h
\nHôte : Michel Lafleur
\nCette conférence sera
  prononcée (en anglais)par le  Professeur Andrzej J. Kulik du Laborat
 oire de Physique de la Matière Vivante de l'École polytechnique fédéra
 le de Lausanne.
\nRésumé:Classical Infrared Spectroscopy has two limitat
 ions – source power and poor spatial resolution. Major improvements beca
 me available recently. Chemical analysis with high spatial resolution is b
 ringing new information\, when samples are inhomogeneous or when only very
  small quantity is available. Using tunable\, pulsed Infrared (IR) laser a
 s a source and contact AFM as a detector\, one is able to measure local IR
  absorption by measuring the local thermal dilatation of the sample\, and 
 enhancing it using resonances of AFM cantilever. This technique measures r
 eliable IR spectra with spatial resolution downto10 nm [2]. Complementary 
 functional imaging of local IR absorption at fixed wavelength\, proved to 
 be very useful. Simultaneously\, frequency of AFM cantilever in contact wi
 th the sample\, is mapping local stiffness.
\nDuring this talk\, I will de
 scribe the technique and discuss two biological applications: i) Light Har
 vesting Proteins (LHCII) are responsible for photosynthesis. Embedded in a
 rtificial lipid membrane\, they form characteristic pillars\, when extract
 ed from the leaves grown in shadow\, improving efficiency of photosynthesi
 s. On the other hand\, LHCII extracted from leaves grown in sunshine are n
 ot forming pillars\, since enhancement of the efficiency is not needed [1]
 . ii) Amyloids are insoluble aggregates of proteins related to neurodegene
 rative diseases. Several examples of measurements of Amyloid fibrils will 
 be equally presented\, together with an assessment of the technique – na
 noIR [3]. The nanoIR showed that ataxin-3 misfold after it aggregates\, no
 t before as would be expected by current views on aggregation [4]
\n[1] E.
  Janik et al.\, The Plant Cell\, 2013\, 25\, 6\, 2155-2170.
\n[2] W.I: Gru
 szecki et al. Nanoscale\, 2015\,7\, 14659-14662 DOI: 10.1039/c5nr03090k
\n
 [3] T. Müller et al. Lab Chip\, 2014\, 14\, 1315-1319
\n[4] F.S. Ruggeri 
 et al.\, Nature Communications 2015\, 6\, 7831\, doi:10.1038/ncomms8831
\n
 Information supplémentaire
\nAnnonce PDF de la conférence
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