BEGIN:VCALENDAR
VERSION:2.0
PRODID:https://murmitoyen.com/events/vanille/udem/
X-WR-TIMEZONE:America/Montreal
BEGIN:VEVENT
UID:69e2d22809d7f
DTSTAMP:20260417T203656
DTSTART:20111116T113000
SEQUENCE:0
TRANSP:OPAQUE
DTEND:20111116T130000
URL:https://murmitoyen.com/events/vanille/udem/detail/68531
LOCATION:Université de Montréal - Pavillon Roger-Gaudry\, 2900\, chemin d
 e la Tour\, Montréal\, QC\, Canada\, H3T 1J6
SUMMARY:Conférence du professeur Edgar Arriaga (Minnesota) *** ANNULÉE **
 *
DESCRIPTION:Titre : Subcellular Analytical Strategies.Attention\, cette con
 férence qui devait être prononcée par le professeur Edgar Arriaga du d
 épartement de chimie de l'University of Minnesota doit être annulée à 
 la toute dernière minute pour des raisons imprévues\, en dehors de notre
  contrôle. En tout état de cause\, notre conférencier invité reviendra
  à Montréal dans le courant de la session d'automne 2012 (détails à d
 éterminer).Résumé : Chemical cytometry opened up exciting opportunitie
 s for monitoring multiple chemical species in single cells and understand 
 cellular heterogeneity. Such heterogeneity results partially from the comp
 lex subcellular organization of the cell\, which is defined by thousands o
 f organelles and their interactions. Furthermore\, organelles are themselv
 es highly heterogeneous\, plastic\, and typically of sub-micrometer dimens
 ions. These characteristics make organelles difficult to investigate with 
 conventional analytical technologies. This presentation describes our cur
 rent work aiming at developing and applying bioanalytical methods to analy
 ze and purify organelles. Three strategies will be highlighted: (1) Biolog
 ical imaging of mitochondrial subpopulations and their carbonylation statu
 s\, (2) affinity purification of subcellular compartments\, and (3) capill
 ary electrophoretic separations of individual organelles. The  first str
 ategy focuses on the analysis of organelles in situ. After dual immunolabe
 ling and quantitative imaging of rat skeletal muscle cross-sections\, imag
 es were analyzed using a semi-automated Image J protocol to determine carb
 onylation levels in subsarcolemmal and interfibrillar mitochondria. Age-re
 lated changes in the carbonylation levels of mitochondria will be discusse
 d. The second strategy is aimed at understanding the biological function 
 of specific organelle types. Mitochondria and peroxisomes usually co-purif
 y. Peroxisomes were magnetically immunopurified and then their ability to 
 process fatty acids and the anti-cancer drug Doxorubicin was investigated.
  Ongoing efforts to immunopurify mitochondria will also be presented. The
  third strategy refers to the use of capillary electrophoretic separations
  of organelles to understand subcellular heterogeneity. Mitochondria utili
 ze cytoskeleton as a scaffold for movement within the cell. Individual org
 anelle analysis by capillary electrophoresis with dual laser-induced fluor
 escence detection revealed that mitochondria are either cytoskeleton-bound
  or –free organelles. The relevance of these studies to abnormal mitocho
 ndria will be discussed. Information supplémentaire
END:VEVENT
BEGIN:VTIMEZONE
TZID:America/Montreal
X-LIC-LOCATION:America/Montreal
END:VTIMEZONE
END:VCALENDAR