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UID:69df31476301a
DTSTAMP:20260415T023343
DTSTART:20141028T130000
SEQUENCE:0
TRANSP:OPAQUE
DTEND:20141028T143000
URL:https://murmitoyen.com/events/vanille/udem/detail/510361
LOCATION:Université de Montréal - Pavillon J.-Armand-Bombardier\, 5155\, 
 chemin de la rampe \, Montréal\, QC\, Canada\, H3T 2B2
SUMMARY:Conférence de Karen Faulds\, Ph.D. (Strathclyde)
DESCRIPTION:Titre : Multiplexed and Sensitive Molecular Diagnostics Using S
 ERRS.Salle : Pavillon J.-A.-Bombardier\, salle 1035 à 13 hHôte : Profess
 eur Jean-François MassonCette conférence sera prononcée (en anglais) p
 ar Karen Faulds\, Ph.D.\, du Département de chimie pure et appliquée de 
 l'Université de Strathclyde (Écosse).Résumé : Surface enhanced resonan
 ce Raman scattering (SERRS) is an analytical technique with several advant
 ages over competitive techniques in terms of improved sensitivity and mult
 iplexing. We have made great progress in the development of SERRS as a qua
 ntitative analytical method\, in particular for the detection of DNA. Howe
 ver\, the lack of quantitative data relating to real examples has prevente
 d more widespread adoption of the technique. Detection of specific DNA seq
 uences is central to modern molecular biology and also to molecular diagno
 stics where identification of a particular disease is based on nucleic aci
 d identification. Many methods exist and fluorescence spectroscopy dominat
 es the detection technologies employed with different assay formats. Anoth
 er advantage of SERRS over existing detection techniques is that of the ab
 ility to multiplex which is limited when using techniques such as fluoresc
 ence. We have clearly demonstrated the ability to identify the presence of
  a mixture of 6 analytes in solution using data analysis techniques. Here 
 we demonstrate the development of new bioassays based upon SERRS which hav
 e been used successfully for the detection of bacterial pathogens using mo
 dified SERRS active probes. The probes have been designed to give a specif
 ic SERRS response resulting in discernable differences in the SERRS which 
 can be correlated to a specific DNA hybridisation event.Information suppl
 émentaire
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