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DTSTAMP:20260406T093707
DTSTART:20110323T113000
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DTEND:20110323T130000
URL:https://murmitoyen.com/events/vanille/udem/detail/37056
LOCATION:Université de Montréal - Pavillon Roger-Gaudry\, 2900\, chemin d
 e la Tour\, Montréal\, QC\, Canada\, H3T 1J6
SUMMARY:Conférence du professeur Edgar Arriaga (Minnesota): ANNULÉE
DESCRIPTION:Titre : Subcellular Analytical Strategies.Pour des raisons en d
 ehors de notre contrôle\, la conférence du professeur Edgar Arriaga des
  départements de chimie et de génie biomédical de l'University of Minne
 sota doit être annulée. Elle sera reportée en temps et lieu (détails 
 à venir)Résumé : Chemical cytometry opened up exciting opportunities f
 or monitoring multiple chemical species in single cells and understand cel
 lular heterogeneity. Such heterogeneity results partially from the complex
  subcellular organization of the cell\, which is defined by thousands of o
 rganelles and their interactions. Furthermore\, organelles are themselves 
 highly heterogeneous\, plastic\, and typically of sub-micrometer dimension
 s. These characteristics make organelles difficult to investigate with con
 ventional analytical technologies. This presentation describes our curren
 t work aiming at developing and applying bioanalytical methods to analyze 
 and purify organelles. Three strategies will be highlighted: (1) Biologica
 l imaging of mitochondrial subpopulations and their carbonylation status\,
  (2) affinity purification of subcellular compartments\, and (3) capillary
  electrophoretic separations of individual organelles. The  first strate
 gy focuses on the analysis of organelles in situ. After dual immunolabelin
 g and quantitative imaging of rat skeletal muscle cross-sections\, images 
 were analyzed using a semi-automated Image J protocol to determine carbony
 lation levels in subsarcolemmal and interfibrillar mitochondria. Age-relat
 ed changes in the carbonylation levels of mitochondria will be discussed.
  The second strategy is aimed at understanding the biological function of
  specific organelle types. Mitochondria and peroxisomes usually co-purify.
  Peroxisomes were magnetically immunopurified and then their ability to pr
 ocess fatty acids and the anti-cancer drug Doxorubicin was investigated. O
 ngoing efforts to immunopurify mitochondria will also be presented. The t
 hird strategy refers to the use of capillary electrophoretic separations o
 f organelles to understand subcellular heterogeneity. Mitochondria utilize
  cytoskeleton as a scaffold for movement within the cell. Individual organ
 elle analysis by capillary electrophoresis with dual laser-induced fluores
 cence detection revealed that mitochondria are either cytoskeleton-bound o
 r –free organelles. The relevance of these studies to abnormal mitochond
 ria will be discussed. Information supplémentaire
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TZID:America/Montreal
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